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Product Profile
Product Name
iNOS antibody
Antibody Type
Primary Antibodies
Product description
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Mouse monoclonal antibody [4E5] to iNOSNitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission ａnd antimicrobial ａnd antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver ａnd is inducible by a combination of lipopolysaccharide ａnd certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Immunogen
The immunogen corresponding to human NOS2 expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4E5
Host Species
Mouse
Tested Applications
ELISAFACSIHC-PWB
ELISA: 1/10000.:
Species Reactivity
HumanMouseRat
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration)
Purification
Target Information
Gene Synonyms
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NOS2
NOS2A
INOS
HEP-NOS
NOS
Target
iNOS
Molecular Weight(MW)
131 kDa (note)
Application
Application
- Western blot analysis using NOS2 mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5)and MCF-7 (6) cell lysate.
Application
- Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NOS2 mouse mAb with DAB staining.
Application
- Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Application
- Flow cytometric analysis of MCF-7 cells using NOS2 mouse mAb (green) ａnd negative control (red).
Application Notes
ELISA: 1/10000.:
Additional Information
Form
Liquid
Storage Instructions
4°C; -20°C for long term storage
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide ａnd 0.5% protein stabilizer.
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