ANXA1 Antibody Catalog Number：OM271186

Product Profile

Product Name

ANXA1 Antibody

Antibody Type

Primary Antibodies

Product description

More

ANXA1 encodes a protein that belongs to a family of Ca (2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 ａnd are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins ａnd leukotrienes, annexin I may have potential anti-inflammatory activity.1) Ernst, S., et al., (2004) J. Immunol. 172 (12), 7669-7676.

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANXA1.

Key Feature

Clonality

Polyclonal

Host Species

Rabbit

Tested Applications

ELISAIHCWB

ANXA1 antibody can be used for detection of ANXA1 by ELISA at 1:62500. ANXA1 antibody can be used for detection of ANXA1 by western blot at 1.25 μg/mL, ａnd HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:

Species Reactivity

Human

Concentration

1 mg/ml

Purification

Protein A

Target Information

Gene Symbol

ANXA1

Alternative Names

ANXA1
ANX1
LPC1

Molecular Weight(MW)

38 kDa

Database Links

Entrez Gene

301

Protein Accession

NP_000691

Application

Application

• Antibody used in WB on mouse fibroblasts at 1:600.

Application

• Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody ａnd Lane B: Primary Antibody + Blocking Peptide ).

Application

• Antibody used in IHC on Human Intestine.

Application

• Antibody used in WB on Human HepG2 at 1.25 ug/ml.

Positive Control

1) Cat. No. 1211 - HepG2 Cell Lysate

Application Notes

ANXA1 antibody can be used for detection of ANXA1 by ELISA at 1:62500. ANXA1 antibody can be used for detection of ANXA1 by western blot at 1.25 μg/mL, ａnd HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:

Additional Information

Form

Liquid

Storage Instructions

For short periods of storage (days) store at 4˚C. For longer periods of storage, store ANXA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Storage Buffer

IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt